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12 results

Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons

Journal Article
Barnes Jr., E. M., Baumgartner, B. J., Harvey, R. J., & Darlison, M. G. (1994)
Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons. Molecular Brain Research, 26(1-2), 9-17. https://doi.org/10.1016/0169-328x%2894%2990068-x
Research report We have used quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to analyze the expression of {GABAA} receptor subunit genes in cultured neu...

Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique

Journal Article
Lasham, A., & Darlison, M. G. (1993)
Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique. Molecular and Cellular Probes, 7(1), 67-73. doi:10.1006/mcpr.1993.1009
We described an improved method for directly sequencing lambda (λ) DNA that has been isolated from either crude cleared lysates or plate lysates. This protocol does not requir...

Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate

Journal Article
Darlison, M. G., Bermudez, I., Van Minnen, J., Zimmermann, C., Amar, M., Dautzenberg, F. M., …Harvey, R. J. (1993)
Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate. Netherlands Journal of Zoology, 44(3), 473-485. https://doi.org/10.1163/156854293x00557
Ligand-gated ion channels (also called ionotropic receptors) are a class of receptors which possess an intrinsic ion pore, selective for either cations or anions, that can be ...

Localization of GABAA receptor α-subunit mRNAs in relation to receptor subtypes

Journal Article
Wisden, W., Morris, B. J., Darlison, M. G., Hunt, S. P., & Barnard, E. A. (1989)
Localization of GABAA receptor α-subunit mRNAs in relation to receptor subtypes. Molecular Brain Research, 5(4), 305-310. https://doi.org/10.1016/0169-328x%2889%2990065-x
The distribution of 3 {GABAA} receptor α-subunit mRNAs in various regions of bovine brain has been investigated using in situ hybridization. Whereas the α2- and α3-transcripts...

Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase ofEscherichia coli

Journal Article
Darlison, M. G., & Guest, J. R. (1984)
Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase ofEscherichia coli. Biochemical Journal, 223(2), 507-517. https://doi.org/10.1042/bj2230507
The nucleotide sequence of a 961 base-pair segment of DNA containing the sdhB gene, which encodes the iron-sulphur protein subunit of the E. coli succinate dehydrogenase, has ...

Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli

Journal Article
Wood, D., Darlison, M. G., Wilde, R. J., & Guest, J. R. (1984)
Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli. The biochemical journal, 222(2), 519-534. https://doi.org/10.1042/bj2220519
The nucleotide sequence of a 3614 base-pair segment of DNA containing the sdhA gene, encoding the flavoprotein subunit of succinate dehydrogenase of Escherichia coli, and two ...

Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase

Journal Article
Spencer, M. E., Darlison, M. G., Stephens, P. E., Duckenfield, I. K., & Guest, J. R. (1984)
Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase. European journal of biochemistry / FEBS, 141(2), 361-374. https://doi.org/10.1111/j.1432-1033.1984.tb08200.x
The nucleotide sequence of the sucB gene, which encodes the dihydrolipoamide succinyltransferase component (E2o) of the 2‐oxoglutarate dehydrogenase complex of Escherichia col...

Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12

Journal Article
Darlison, M. G., Spencer, M. E., & Guest, J. R. (1984)
Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12. European journal of biochemistry / FEBS, 141(2), 351-359. https://doi.org/10.1111/j.1432-1033.1984.tb08199.x
The nucleotide sequence of a 3180‐base‐pair segment of DNA, containing the sucA gene encoding the 2‐oxoglutarate dehydrogenase component (E1o) of the 2‐oxoglutarate dehydrogen...

Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12

Journal Article
Stephens, P. E., Lewis, H. M., Darlison, M. G., & Guest, J. R. (1983)
Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12. European journal of biochemistry / FEBS, 135(3), 519-527. https://doi.org/10.1111/j.1432-1033.1983.tb07683.x
The nucleotide sequence of a 1980‐base‐pair segment of DNA, containing the lpd gene encoding the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase complex o...

The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Dihydrolipoamide Acetyltransferase Component

Journal Article
Stephens, P. E., Darlison, M. G., Lewis, H. M., & Guest, J. R. (1983)
The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Dihydrolipoamide Acetyltransferase Component. European journal of biochemistry / FEBS, 133(3), 481-489. https://doi.org/10.1111/j.1432-1033.1983.tb07490.x
The nucleotide sequence of the aceF gene, which encodes the dihydrolipoamide acetyltransferase component (E2) of the pyruvate dehydrogenase complex of Escherichia coli K12, ha...