Death-associated protein kinase (DAPK) and signal transduction: additional roles beyond cell death: DAPK and signal transduction
Journal Article
Lin, Y., Hupp, T. R., & Stevens, C. (2009)
Death-associated protein kinase (DAPK) and signal transduction: additional roles beyond cell death: DAPK and signal transduction. FEBS Journal, 277(1), (48-57). ISSN 1742-464X
Death-associated protein kinase (DAPK) is a stress-regulated protein
kinase that mediates a range of processes, including signal-induced cell death and autophagy. Although the...
ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1: ATP stimulated inhibition of E2F1 by MDM2
Journal Article
Stevens, C., Pettersson, S., Wawrzynow, B., Wallace, M., Ball, K., Zylicz, A., & Hupp, T. R. (2008)
ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1: ATP stimulated inhibition of E2F1 by MDM2. FEBS Journal, 275(19), 4875-4886. https://doi.org/10.1111/j.1742-4658.2008.06627.x
Murine double minute 2 (MDM2) protein exhibits many diverse biochemical functions on the tumour suppressor protein p53, including transcriptional suppression and E3 ubiquitin ...
Novel insights into DAPK autophagic signalling using peptide aptamer combinatorial protein-interaction screens
Journal Article
Stevens, C. & Hupp, T. R. (2008)
Novel insights into DAPK autophagic signalling using peptide aptamer combinatorial protein-interaction screens. Autophagy. 4(4), 531-533. doi:10.4161/auto.5940. ISSN 1554-8627
DAPK represents a relatively unique enzyme in the protein kinase superfamily whose major biological functions are linked to both autophagy and signal-mediated apoptosis. Howev...
Senescent T-lymphocytes are mobilised into the peripheral blood compartment in young and older humans after exhaustive exercise
Journal Article
Simpson, R. J., Cosgrove, C., Ingram, L. A., Florida-James, G. D., Whyte, G. P., Pircher, H., & Guy, K. (2008)
Senescent T-lymphocytes are mobilised into the peripheral blood compartment in young and older humans after exhaustive exercise. Brain, Behavior, and Immunity, 22(4), 544-551. https://doi.org/10.1016/j.bbi.2007.11.002
Senescent T-lymphocytes are antigen-experienced cells that express the killer-cell lectin-like receptor G1 (KLRG1) and/or CD57; fail to clonally expand following further antig...
Parasitic helminths tip the balance: potential anti-inflammatory therapies
Journal Article
Proudfoot, L. (2004)
Parasitic helminths tip the balance: potential anti-inflammatory therapies. Immunology, 113(4), 438-440. https://doi.org/10.1111/j.1365-2567.2004.01996.x
Parasitic helminths are worms that are classified within the phyla Nematoda (roundworms) and Platyhelminthes (flatworms) (see Table 1). Some nematode species, Filariae being a...
Chk2 activates E2F-1 in response to DNA damage
Journal Article
Stevens, C., Smith, L., & La Thangue, N. B. (2003)
Chk2 activates E2F-1 in response to DNA damage. Nature Cell Biology, 5(5), 401-409. https://doi.org/10.1038/ncb974
The E2F-1 transcription factor is regulated during cell cycle progression and induced by cellular stress, such as DNA damage. We report that checkpoint kinase 2 (Chk2) regulat...
Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons
Journal Article
Barnes Jr., E. M., Baumgartner, B. J., Harvey, R. J., & Darlison, M. G. (1994)
Developmental up-regulation and agonist-dependent down-regulation of GABAA receptor subunit mRNAs in chick cortical neurons. Molecular Brain Research, 26(1-2), 9-17. https://doi.org/10.1016/0169-328x%2894%2990068-x
Research report
We have used quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to analyze the expression of {GABAA} receptor subunit genes in cultured neu...
Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique
Journal Article
Lasham, A., & Darlison, M. G. (1993)
Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique. Molecular and Cellular Probes, 7(1), 67-73. doi:10.1006/mcpr.1993.1009
We described an improved method for directly sequencing lambda (λ) DNA that has been isolated from either crude cleared lysates or plate lysates. This protocol does not requir...
Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate
Journal Article
Darlison, M. G., Bermudez, I., Van Minnen, J., Zimmermann, C., Amar, M., Dautzenberg, F. M., …Harvey, R. J. (1993)
Molecular Characterisation and Functional Expression of Molluscan Ion-Channel Receptors That Can Be Activated By Either γ-Aminobutyric Acid or L-Glutamate. Netherlands Journal of Zoology, 44(3), 473-485. https://doi.org/10.1163/156854293x00557
Ligand-gated ion channels (also called ionotropic receptors) are a class of receptors which possess an intrinsic ion pore, selective for either cations or anions, that can be ...
Localization of GABAA receptor α-subunit mRNAs in relation to receptor subtypes
Journal Article
Wisden, W., Morris, B. J., Darlison, M. G., Hunt, S. P., & Barnard, E. A. (1989)
Localization of GABAA receptor α-subunit mRNAs in relation to receptor subtypes. Molecular Brain Research, 5(4), 305-310. https://doi.org/10.1016/0169-328x%2889%2990065-x
The distribution of 3 {GABAA} receptor α-subunit mRNAs in various regions of bovine brain has been investigated using in situ hybridization. Whereas the α2- and α3-transcripts...