The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase
Journal Article
Boyd, G. W., Drew, M., Ward, S., Baird, M., Graham, S. M., & Connaboy, C. (2018)
The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase. Applied Physiology, Nutrition, and Metabolism, https://doi.org/10.1139/apnm-2018-0449
Branched-chain amino acids (BCAA) are used as nutritional support for patients with a range of conditions including liver cirrhosis and in-born errors of amino acid metabolism...
Sost deficiency does not alter bone’s lacunar or vascular porosity in mice
Journal Article
Mosey, H., Núñez, J. A., Goring, A., Clarkin, C. E., Staines, K. A., Lee, P. D., …Javaheri, B. (2017)
Sost deficiency does not alter bone’s lacunar or vascular porosity in mice. Frontiers in Materials, 4, https://doi.org/10.3389/fmats.2017.00027
SCLEROSTIN (Sost) is expressed predominantly in osteocytes acting as a negative regulator of bone formation. In humans, mutations in the SOST gene lead to skeletal overgrowth ...
A distinctive patchy osteomalacia characterises Phospho1
-deficient mice
Journal Article
-deficient mice. Journal of Anatomy, 231(2), 298-308. https://doi.org/10.1111/joa.12628)
-deficient mice. Journal of Anatomy, 231(2), 298-308. https://doi.org/10.1111/joa.12628
The phosphatase PHOSPHO1 is involved in the initiation of biomineralisation. Bones in Phospho1 KO mice show histological osteomalacia with frequent bowing of long bones and sp...
Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model
Journal Article
Staines, K. A., Madi, K., Mirczuk, S. M., Parker, S., Burleigh, A., Poulet, B., …Pitsillides, A. A. (2016)
Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model. Arthritis and Rheumatology, 68(4), 880-891. https://doi.org/10.1002/art.39508
OBJECTIVE:
To explore whether aberrant transient chondrocyte behaviors occur in the joints of STR/Ort mice (which spontaneously develop osteoarthritis [OA]) and whether they a...
E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models
Journal Article
Staines, K. A., Prideaux, M., Allen, S., Buttle, D. J., Pitsillides, A. A., & Farquharson, C. (2016)
E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models. Journal of Cellular Physiology, 231(6), 1392-1404. https://doi.org/10.1002/jcp.25282
The transmembrane glycoprotein E11 is considered critical in early osteoblast–osteocyte transitions (osteocytogenesis), however its function and regulatory mechanisms are stil...
Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing
Journal Article
Zaman, G., Farquharson, C., Staines, K. A., Newton, P. T., Dudhia, J., Chenu, C., …Dhoot, G. K. (2016)
Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing. Histochemistry and Cell Biology, 145(1), 67-79. https://doi.org/10.1007/s00418-015-1365-8
SULF1/SULF2 enzymes regulate cell signalling that impacts the growth and differentiation of many tissues. To determine their possible role in cartilage and bone growth or repa...
miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION
Journal Article
Mackenzie, N. C. W., Staines, K. A., Zhu, D., Genever, P., & MacRae, V. E. (2014)
miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION. Cell Biochemistry and Function, 32(2), 209-216. https://doi.org/10.1002/cbf.3005
Vascular calcification shares many similarities with skeletal mineralisation and involves the phenotypic trans-differentiation of vascular smooth muscle cells (VSMCs) to osteo...
Death-associated protein kinase (DAPK) and signal transduction: additional roles beyond cell death: DAPK and signal transduction
Journal Article
Lin, Y., Hupp, T. R., & Stevens, C. (2009)
Death-associated protein kinase (DAPK) and signal transduction: additional roles beyond cell death: DAPK and signal transduction. FEBS Journal, 277(1), (48-57). ISSN 1742-464X
Death-associated protein kinase (DAPK) is a stress-regulated protein
kinase that mediates a range of processes, including signal-induced cell death and autophagy. Although the...
ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1: ATP stimulated inhibition of E2F1 by MDM2
Journal Article
Stevens, C., Pettersson, S., Wawrzynow, B., Wallace, M., Ball, K., Zylicz, A., & Hupp, T. R. (2008)
ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1: ATP stimulated inhibition of E2F1 by MDM2. FEBS Journal, 275(19), 4875-4886. https://doi.org/10.1111/j.1742-4658.2008.06627.x
Murine double minute 2 (MDM2) protein exhibits many diverse biochemical functions on the tumour suppressor protein p53, including transcriptional suppression and E3 ubiquitin ...
Novel insights into DAPK autophagic signalling using peptide aptamer combinatorial protein-interaction screens
Journal Article
Stevens, C. & Hupp, T. R. (2008)
Novel insights into DAPK autophagic signalling using peptide aptamer combinatorial protein-interaction screens. Autophagy. 4(4), 531-533. doi:10.4161/auto.5940. ISSN 1554-8627
DAPK represents a relatively unique enzyme in the protein kinase superfamily whose major biological functions are linked to both autophagy and signal-mediated apoptosis. Howev...