Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation.
Journal Article
Buttle, D. J., Pitsillides, A. A., Staines, K. A., Javaheri, B., Hohenstein, P., Fleming, R., …Farquharson, C. (2017)
Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation. Journal of Cellular Physiology, 232(11), 3006-3019. https://doi.org/10.1002/jcp.25999
The transmembrane glycoprotein E11/Podoplanin (Pdpn) has been implicated in the initial stages of osteocyte differentiation. However, its precise function and regulatory mecha...
Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity
Journal Article
Poulet, B., Liu, K., Plumb, D., Vo, P., Shah, M., Staines, K., …Bou-Gharios, G. (2016)
Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity. PLOS ONE, 11(12), https://doi.org/10.1371/journal.pone.0167971
Bone development and length relies on the growth plate formation, which is dependent on degradative enzymes such as MMPs. Indeed, deletion of specific members of this enzyme f...
The STR/ort mouse model of spontaneous osteoarthritis – an update
Journal Article
Staines, K. A., Poulet, B., Wentworth, D. N., & Pitsillides, A. A. (2017)
The STR/ort mouse model of spontaneous osteoarthritis – an update. Osteoarthritis and Cartilage, 25(6), 802-808. https://doi.org/10.1016/j.joca.2016.12.014
Osteoarthritis is a degenerative joint disease and a world-wide healthcare burden. Characterized by cartilage degradation, subchondral bone thickening and osteophyte formation...
The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures
Journal Article
Houston, D. A., Myers, K., MacRae, V. E., Staines, K. A., & Farquharson, C. (2016)
The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures. Calcified Tissue International, 99(5), 510-524. https://doi.org/10.1007/s00223-016-0176-9
Sustained exposure to high levels of parathyroid hormone (PTH), as observed in hyperparathyroidism, are catabolic to bone. The increase in the RANKL/OPG ratio in response to c...
Models of ex vivo explant cultures: applications in bone research
Journal Article
Marino, S., Staines, K. A., Brown, G., Howard-Jones, R. A., & Adamczyk, M. (2016)
Models of ex vivo explant cultures: applications in bone research. BoneKEy Reports, 5, 818. https://doi.org/10.1038/bonekey.2016.49
Ex vivo explant culture models are powerful tools in bone research. They allow investigation of bone and cartilage responses to specific stimuli in a controlled manner that cl...
Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model
Journal Article
Staines, K. A., Madi, K., Mirczuk, S. M., Parker, S., Burleigh, A., Poulet, B., …Pitsillides, A. A. (2016)
Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model. Arthritis and Rheumatology, 68(4), 880-891. https://doi.org/10.1002/art.39508
OBJECTIVE:
To explore whether aberrant transient chondrocyte behaviors occur in the joints of STR/Ort mice (which spontaneously develop osteoarthritis [OA]) and whether they a...
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
Journal Article
Houston, D. A., Staines, K. A., MacRae, V. E., & Farquharson, C. (2016)
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification. Journal of Visualized Experiments, 118(118), https://doi.org/10.3791/54978
The fundamental process of endochondral ossification is under tight regulation in the healthy individual so as to prevent disturbed development and/or longitudinal bone growth...
New developments in osteoarthritis and cartilage biology
Journal Article
Poulet, B., & Staines, K. A. (2016)
New developments in osteoarthritis and cartilage biology. Current Opinion in Pharmacology, 28, 8-13. https://doi.org/10.1016/j.coph.2016.02.009
Osteoarthritis (OA) is a degenerative joint disease and the most common form of arthritis. Characterised by articular cartilage loss, subchondral bone thickening and osteophyt...
E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models
Journal Article
Staines, K. A., Prideaux, M., Allen, S., Buttle, D. J., Pitsillides, A. A., & Farquharson, C. (2016)
E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models. Journal of Cellular Physiology, 231(6), 1392-1404. https://doi.org/10.1002/jcp.25282
The transmembrane glycoprotein E11 is considered critical in early osteoblast–osteocyte transitions (osteocytogenesis), however its function and regulatory mechanisms are stil...
Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing
Journal Article
Zaman, G., Farquharson, C., Staines, K. A., Newton, P. T., Dudhia, J., Chenu, C., …Dhoot, G. K. (2016)
Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing. Histochemistry and Cell Biology, 145(1), 67-79. https://doi.org/10.1007/s00418-015-1365-8
SULF1/SULF2 enzymes regulate cell signalling that impacts the growth and differentiation of many tissues. To determine their possible role in cartilage and bone growth or repa...