Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters
Journal Article
Harvey, R. J., Harder, S., & Darlison, M. G. (1998)
Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters. Technical tips online, 3(1), 150-152. https://doi.org/10.1016/S1366-2120%2808%2970126-0
Many laboratories still find the direct sequencing of large DNA templates (i.e. lambda, cosmid and bacteriophage P1 clones) problematic. To date, only limited amounts of seque...
In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain
Journal Article
Albrecht, B. E., Breitenbach, U., Stühmer, T., Harvey, R. J., & Darlison, M. G. (1997)
In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain. European Journal of Neuroscience, 9(11), 2414-2422. https://doi.org/10.1111/j.1460-9568.1997.tb01658.x
The pharmacological properties of homo-oligomeric channels formed by the GABA type A receptor-like ρl and ρ2 polypeptides are very reminiscent of those of the GABA type C rece...
Candidate gene study of eight GABAA receptor subunits in panic disorder
Journal Article
Darlison, M. G., Bailey, M., Johnson, K., Wang, Z., Crowe, R., Albrecht, B., …Zoega, T. (1997)
Candidate gene study of eight GABAA receptor subunits in panic disorder. American Journal of Psychiatry, 154(8), 1096-1100. https://doi.org/10.1176/ajp.154.8.1096
OBJECTIVE: gamma-Aminobutyric acid type A (GABAA) receptor subunit genes are candidate genes for panic disorder. Benzodiazepine agonists acting at this receptor can suppress p...
Opioid receptors from a lower vertebrate (Catostomus commersoni): Sequence, pharmacology, coupling to a G-protein-gated inward-rectifying potassium channel (GIRK1), and evolution
Journal Article
Darlison, M. G., Greten, F. R., Harvey, R. J., Kreienkamp, H., Stühmer, T., Zwiers, H., …Richter, D. (1997)
Opioid receptors from a lower vertebrate (Catostomus commersoni): Sequence, pharmacology, coupling to a G-protein-gated inward-rectifying potassium channel (GIRK1), and evolution. Proceedings of the National Academy of Sciences, 94(15), 8214-8219
The molecular evolution of the opioid receptor family has been studied by isolating cDNAs that encode six distinct opioid receptor-like proteins from a lower vertebrate, the t...
In Situ Hybridization Localization of the GABAA Receptor β2S- and β2L-Subunit Transcripts Reveals Cell-Specific Splicing of Alternate Cassette Exons
Journal Article
Harvey, R., & Darlison, M. (1997)
In Situ Hybridization Localization of the GABAA Receptor β2S- and β2L-Subunit Transcripts Reveals Cell-Specific Splicing of Alternate Cassette Exons. Neuroscience, 77(2), 361-369. https://doi.org/10.1016/S0306-4522%2896%2900467-8
We have recently described two variants of the chicken {GABAA} receptor β2 subunit which arise by alternative splicing of the corresponding primary gene transcript. The long f...
Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes
Journal Article
Harvey, R. J., Stühmer, T., van Minnen, J., & Darlison, M. G. (1997)
Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes. Neuroscience Research Communications, 20(1), 31-40. https://doi.org/10.1002/%28SICI%291520-6769%28199701%2920%3A13.0.CO%3B2-6
We report the isolation of molluscan (Lymnaea stagnalis) partial complementary DNAs (cDNAs) that encode two new putative glutamate-gated cation-channel polypeptides. These pro...
GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness
Book Chapter
Darlison, M. G., & Harvey, R. J. (1996)
GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness. In Molecular Biology of Membrane Transport Disorders, 169-180. Springer Verlag. https://doi.org/10.1007/978-1-4613-1143-0_9
Rapid chemical communication between cells in the vertebrate central nervous system is mediated by ligand-gated ion-channel receptors (also called ionotropic receptors), which...
Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells
Journal Article
Bahn, S., Harvey, R. J., Darlison, M. G., & Wisden, W. (1996)
Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells. Journal of Neurochemistry, 66(5), 1810-1818. https://doi.org/10.1046/j.1471-4159.1996.66051810.x
The γ-aminobutyric acid type A receptor cDNAs encoding the α6 subunit homologues from chicken and goldfish have been cloned and sequenced. These proteins exhibit 83 and 75% id...
Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5
Journal Article
Sander, T., Hildmann, T., Janz, D., Wienker, T. F., Bianchi, A., Bauer, G., …Darlison, M. G. (1996)
Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5. Epilepsy Research, 23(3), 235-244. https://doi.org/10.1016/0920-1211%2895%2900098-4
Hereditary factors play a major role in the etiology of idiopathic generalized epilepsies (IGEs). The pivotal function of ionotropic γ-aminobutyric acid type A receptors (GABR...
Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA
Journal Article
Bhandal, N. S., Ramsey, R. L., Harvey, R. J., Darlison, M. G., & Usherwood, P. N. R. (1995)
Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA. Invertebrate Neuroscience, 1(3), 267-272. https://doi.org/10.1007/bf02211028
We have previously described the isolation of a complementary DNA (cDNA) from the freshwater molluscLymnaea stagnalis encoding a polypeptide that exhibits ∼50{%} identity to t...