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11 results

The Production of a Stably Transformed Insect Cell Line Expressing An Invertebrate GABA A Receptor β-Subunit

Journal Article
Smith, L. A., Amar, M., Harvey, R. J., Darlison, M. G., Earley, F. G. P., Beadle, D. J., …Bermudez, I. (1995)
The Production of a Stably Transformed Insect Cell Line Expressing An Invertebrate GABA A Receptor β-Subunit. Journal of Receptors and Signal Transduction, 15(1-4), 33-41. https://doi.org/10.3109/10799899509045205
We have produced a stable insect cell line derived from Spodoptera frugiperda (Sf9) cells expressing a cDNA encoding a β-subunit of the Lymnaea stagnalis GABAA receptor. The c...

Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique

Journal Article
Lasham, A., & Darlison, M. G. (1993)
Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique. Molecular and Cellular Probes, 7(1), 67-73. doi:10.1006/mcpr.1993.1009
We described an improved method for directly sequencing lambda (λ) DNA that has been isolated from either crude cleared lysates or plate lysates. This protocol does not requir...

Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors

Journal Article
Atkinson, A. E., Bermudez, I., Darlison, M. G., Barnard, E. A., Earley, F. G. P., Possee, R. D., …King, L. A. (1992)
Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors. NeuroReport, 3(7), 597-600. https://doi.org/10.1097/00001756-199207000-00013
We have constructed recombinant baculoviruses containing cDNAs encoding either the [alpha]1- or the [beta]1-subunit of the bovine GABAA receptor. In Spodoptera frugiperda (IPL...

Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase ofEscherichia coli

Journal Article
Darlison, M. G., & Guest, J. R. (1984)
Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase ofEscherichia coli. Biochemical Journal, 223(2), 507-517. https://doi.org/10.1042/bj2230507
The nucleotide sequence of a 961 base-pair segment of DNA containing the sdhB gene, which encodes the iron-sulphur protein subunit of the E. coli succinate dehydrogenase, has ...

Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli

Journal Article
Wood, D., Darlison, M. G., Wilde, R. J., & Guest, J. R. (1984)
Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli. The biochemical journal, 222(2), 519-534. https://doi.org/10.1042/bj2220519
The nucleotide sequence of a 3614 base-pair segment of DNA containing the sdhA gene, encoding the flavoprotein subunit of succinate dehydrogenase of Escherichia coli, and two ...

Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase

Journal Article
Spencer, M. E., Darlison, M. G., Stephens, P. E., Duckenfield, I. K., & Guest, J. R. (1984)
Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase. European journal of biochemistry / FEBS, 141(2), 361-374. https://doi.org/10.1111/j.1432-1033.1984.tb08200.x
The nucleotide sequence of the sucB gene, which encodes the dihydrolipoamide succinyltransferase component (E2o) of the 2‐oxoglutarate dehydrogenase complex of Escherichia col...

Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12

Journal Article
Darlison, M. G., Spencer, M. E., & Guest, J. R. (1984)
Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12. European journal of biochemistry / FEBS, 141(2), 351-359. https://doi.org/10.1111/j.1432-1033.1984.tb08199.x
The nucleotide sequence of a 3180‐base‐pair segment of DNA, containing the sucA gene encoding the 2‐oxoglutarate dehydrogenase component (E1o) of the 2‐oxoglutarate dehydrogen...

Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12

Journal Article
Stephens, P. E., Lewis, H. M., Darlison, M. G., & Guest, J. R. (1983)
Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12. European journal of biochemistry / FEBS, 135(3), 519-527. https://doi.org/10.1111/j.1432-1033.1983.tb07683.x
The nucleotide sequence of a 1980‐base‐pair segment of DNA, containing the lpd gene encoding the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase complex o...

The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Dihydrolipoamide Acetyltransferase Component

Journal Article
Stephens, P. E., Darlison, M. G., Lewis, H. M., & Guest, J. R. (1983)
The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Dihydrolipoamide Acetyltransferase Component. European journal of biochemistry / FEBS, 133(3), 481-489. https://doi.org/10.1111/j.1432-1033.1983.tb07490.x
The nucleotide sequence of the aceF gene, which encodes the dihydrolipoamide acetyltransferase component (E2) of the pyruvate dehydrogenase complex of Escherichia coli K12, ha...

The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Pyruvate Dehydrogenase Component

Journal Article
Stephens, P. E., Darlison, M. G., Lewis, H. M., & Guest, J. R. (1983)
The Pyruvate Dehydrogenase Complex of Escherichia coli K12. Nucleotide Sequence Encoding the Pyruvate Dehydrogenase Component. European journal of biochemistry / FEBS, 133(1), 155-162. https://doi.org/10.1111/j.1432-1033.1983.tb07441.x
The nucleotide sequence of a 3780‐base pair segment of DNA containing the aceE gene encoding the pyruvate dehydrogenase component (E1) of the pyruvate dehydrogenase complex of...