18 results

Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters

Journal Article
Harvey, R. J., Harder, S., & Darlison, M. G. (1998)
Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters. Technical tips online, 3(1), 150-152. https://doi.org/10.1016/S1366-2120%2808%2970126-0
Many laboratories still find the direct sequencing of large DNA templates (i.e. lambda, cosmid and bacteriophage P1 clones) problematic. To date, only limited amounts of seque...

In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain

Journal Article
Albrecht, B. E., Breitenbach, U., Stühmer, T., Harvey, R. J., & Darlison, M. G. (1997)
In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain. European Journal of Neuroscience, 9(11), 2414-2422. https://doi.org/10.1111/j.1460-9568.1997.tb01658.x
The pharmacological properties of homo-oligomeric channels formed by the GABA type A receptor-like ρl and ρ2 polypeptides are very reminiscent of those of the GABA type C rece...

Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes

Journal Article
Harvey, R. J., Stühmer, T., van Minnen, J., & Darlison, M. G. (1997)
Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes. Neuroscience Research Communications, 20(1), 31-40. https://doi.org/10.1002/%28SICI%291520-6769%28199701%2920%3A13.0.CO%3B2-6
We report the isolation of molluscan (Lymnaea stagnalis) partial complementary DNAs (cDNAs) that encode two new putative glutamate-gated cation-channel polypeptides. These pro...

GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness

Book Chapter
Darlison, M. G., & Harvey, R. J. (1996)
GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness. In Molecular Biology of Membrane Transport Disorders, 169-180. Springer Verlag. https://doi.org/10.1007/978-1-4613-1143-0_9
Rapid chemical communication between cells in the vertebrate central nervous system is mediated by ligand-gated ion-channel receptors (also called ionotropic receptors), which...

Factors affecting silver biosorption by an industrial strain of Saccharomyces cerevisiae

Journal Article
Singleton, I., & Simmons, P. (1996)
Factors affecting silver biosorption by an industrial strain of Saccharomyces cerevisiae. Journal of Chemical Technology and Biotechnology, 65(1), 21-28. https://doi.org/10.1002/%28SICI%291097-4660%28199601%2965%3A13.0.CO%3B2-E
Factors affecting silver biosorption by Saccharomyces cerevisiae biomass, obtained as a waste product from industry, were examined. Maximum removal of silver from solution was...

Biosynthesis of the glycolipid anchor of lipophosphoglycan and the structurally related glycoinositolphospholipids from Leishmania major

Journal Article
Proudfoot, L., Schneider, P., Ferguson, M. A. J., & McConville, M. J. (1995)
Biosynthesis of the glycolipid anchor of lipophosphoglycan and the structurally related glycoinositolphospholipids from Leishmania major. The biochemical journal, 308(1), 45-55. https://doi.org/10.1042/bj3080045
The major macromolecule on the surface of the protozoan parasite Leishmania major is a lipophosphoglycan (LPG) which contains a glycosylphosphatidylinositol glycolipid anchor....

Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis

Journal Article
Hicks, A. A., Bailey, M. E., Riley, B. P., Kamphuis, W., Siciliano, M. J., Johnson, K. J., & Darlison, M. G. (1994)
Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis. Genomics, 20(2), 285-288. https://doi.org/10.1006/geno.1994.1167
GABAA receptors are hetero-oligomeric ion-channel complexes that are composed of combinations of α, β, γ, and δ subunits and play a major role in inhibitory neurotransmission ...

Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit

Journal Article
Glencorse, T. A., Darlison, M. G., Barnard, E. A., & Bateson, A. N. (1993)
Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit. Journal of Neurochemistry, 61(6), 2294-2302. https://doi.org/10.1111/j.1471-4159.1993.tb07473.x
cDNAs have been cloned that encode the chicken (Gallus domesticus) γ-aminobutyric acidA receptor γ1 subunit, the mature sequence of which shares 90, 79, and 69% identity with ...

Rheological spectroscopy of concentrated colloidal dispersions

Journal Article
Williams, P., Williams, D., & Williams, R. (1993)
Rheological spectroscopy of concentrated colloidal dispersions. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 77(1), 75-79. https://doi.org/10.1016/0927-7757%2893%2980101-j
A new instrument, the virtual gap rheometer (VGR) for the determination of dynamic shear moduli (and mechanical relaxation spectra therefrom) at frequencies above 100 Hz is de...

Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms

Journal Article
Proudfoot, L., Kusel, J. R., Smith, H. V., Harnett, W., Worms, M. J., & Kennedy, M. W. (1993)
Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms. Parasitology, 107(01), 107. doi:10.1017/s0031182000079464
All mammalian-parasitic stages of a range of nematode species investigated (Brugia pahangi, Acanthocheilonema viteae, Strongyloides ratti, Nippostrongylus brasiliensis, Trichi...