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Chemical Genetics Approach to Identify Peptide Ligands that Selectively Stimulate DAPK-1 Kinase Activity

Journal Article
Fraser, J. A., & Hupp, T. R. (2007)
Chemical Genetics Approach to Identify Peptide Ligands that Selectively Stimulate DAPK-1 Kinase Activity. Biochemistry, 46(10), 2655-2673. https://doi.org/10.1021/bi061562j
Dissection of signal transduction pathways has been advanced by classic genetic approaches including targeted gene deletion and siRNA-based inhibition of gene product synthesi...

A Germ Line Mutation in the Death Domain of DAPK-1 Inactivates ERK-induced Apoptosis

Journal Article
Stevens, C., Lin, Y., Sanchez, M., Amin, E., Copson, E., White, H., …Hupp, T. (2007)
A Germ Line Mutation in the Death Domain of DAPK-1 Inactivates ERK-induced Apoptosis. Journal of Biological Chemistry, 282(18), 13791-13803. https://doi.org/10.1074/jbc.m605649200
p53 is activated genetically by a set of kinases that are components of the calcium calmodulin kinase superfamily, including CHK2, AMP kinase, and DAPK-1. In dissecting the me...

Alternative Splicing of a 51-Nucleotide Exon that Encodes a Putative Protein Kinase C Phosphorylation Site Generates Two Forms of the Chicken γ-Aminobutyric AcidAReceptor β2 Subunit

Journal Article
Harvey, R. J., Chinchetru, M. A., & Darlison, M. G. (1994)
Alternative Splicing of a 51-Nucleotide Exon that Encodes a Putative Protein Kinase C Phosphorylation Site Generates Two Forms of the Chicken γ-Aminobutyric AcidAReceptor β2 Subunit. Journal of Neurochemistry, 62(1), 10-16. https://doi.org/10.1046/j.1471-4159.1994.62010010.x
Complementary DNAs that encode two forms of the chicken -γ-aminobutyric acid type A (GABAA) receptor β2 subunit have been isolated. These polypeptides differ by the presence (...

Molecular cloning reveals the existence of a fourth γ subunit of the vertebrate brain GABAAreceptor

Journal Article
Harvey, R. J., Kim, H., & Darlison, M. G. (1993)
Molecular cloning reveals the existence of a fourth γ subunit of the vertebrate brain GABAAreceptor. FEBS Letters, 331(3), 211-216. https://doi.org/10.1016/0014-5793%2893%2980339-v
We have isolated a cDNA, from the chicken, that encodes a fourth type of γ subunit of the vertebrate brain GABAA receptor. The mature polypeptide (which we name γ4) displays 6...

A novel invertebrate GABAAreceptor-like polypeptide Sequence and pattern of gene expression

Journal Article
Hutton, M. L., Harvey, R. J., Earley, F. G., Barnard, E. A., & Darlison, M. G. (1993)
A novel invertebrate GABAAreceptor-like polypeptide Sequence and pattern of gene expression. FEBS Letters, 326(1-3), 112-116. https://doi.org/10.1016/0014-5793%2893%2981773-s
A full‐length complementary DNA has been isolated, from the fresh‐water mollusc Lymnaea stagnalis, that encodes a polypeptide (which we have named Ç) that exhibits between 30%...

Invertebrate GABA and glutamate receptors: molecular biology reveals predictable structures but some unusual pharmacologies

Journal Article
Darlison, M. G. (1992)
Invertebrate GABA and glutamate receptors: molecular biology reveals predictable structures but some unusual pharmacologies. Trends in Neurosciences, 15(12), 469-474. https://doi.org/10.1016/0166-2236%2892%2990091-l
Determination of the sequences of invertebrate γ-aminobutyric acid (GABA)-gated and glutamate-gated receptor/ion channels, through the application of recombinant {DNA} methods...

Confirmation of the localization of the human GABAA receptor α1-subunit gene (GABRA1) to distal 5q by linkage analysis

Journal Article
Johnson, K. J., Sander, T., Hicks, A. A., van Marle, A., Janz, D., Mullan, M. J., …Darlison, M. G. (1992)
Confirmation of the localization of the human GABAA receptor α1-subunit gene (GABRA1) to distal 5q by linkage analysis. Genomics, 14(3), 745-748. https://doi.org/10.1016/s0888-7543%2805%2980178-8
The GABAA receptor is the major inhibitory neurotransmitter receptor in the mammalian brain. To date, 14 genes that encode subunits of this receptor have been identified; thes...

GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis

Journal Article
Walsh, C., Hicks, A. A., Sham, P., Castle, D., Hunt, N., Clements, A., …Gill, M. (1992)
GABAA receptor subunit genes as candidate genes for bipolar affective disorder—an association analysis. Psychiatric Genetics, 2(4), 239-248. https://doi.org/10.1097/00041444-199210000-00002
Polymorphic DNA segments can be used as genetic markers to test for allelic association between a disease and a candidate gene locus. Here we report the results of a study of ...

Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor

Journal Article
Zaman, S. H., Harvey, R. J., Barnard, E. A., & Darlison, M. G. (1992)
Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAAreceptor. FEBS Letters, 307(3), 351-354. https://doi.org/10.1016/0014-5793%2892%2980711-o
We have previously reported [(1991) EMBO J. 10, 3239–3245] the sequence of an invertebrate γ‐aminobutyric acid (GABA) type A (GABAA) receptor polypeptide which forms homo‐olig...

Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel

Journal Article
Traupe, H., Ouweland, A. M. V. D., Oost, B. A. V., Vogel, W., Vetter, U., Warren, S. T., …Ropers, H. (1992)
Fine mapping of the human biglycan (BGN) gene within the Xq28 region employing a hybrid cell panel. Genomics, 13(2), 481-483. https://doi.org/10.1016/0888-7543%2892%2990279-2
Human biglycan is a small proteoglycan that is expressed at high levels in the growing skeleton and in human skin at the cell surface of differentiating keratinocytes. The hum...