Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters
Journal Article
Harvey, R. J., Harder, S., & Darlison, M. G. (1998)
Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters. Technical tips online, 3(1), 150-152. https://doi.org/10.1016/S1366-2120%2808%2970126-0
Many laboratories still find the direct sequencing of large DNA templates (i.e. lambda, cosmid and bacteriophage P1 clones) problematic. To date, only limited amounts of seque...
In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain
Journal Article
Albrecht, B. E., Breitenbach, U., Stühmer, T., Harvey, R. J., & Darlison, M. G. (1997)
In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain. European Journal of Neuroscience, 9(11), 2414-2422. https://doi.org/10.1111/j.1460-9568.1997.tb01658.x
The pharmacological properties of homo-oligomeric channels formed by the GABA type A receptor-like ρl and ρ2 polypeptides are very reminiscent of those of the GABA type C rece...
Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes
Journal Article
Harvey, R. J., Stühmer, T., van Minnen, J., & Darlison, M. G. (1997)
Differential patterns of expression of two novel invertebrate (Lymnaea stagnalis) ionotropic glutamate receptor genes. Neuroscience Research Communications, 20(1), 31-40. https://doi.org/10.1002/%28SICI%291520-6769%28199701%2920%3A13.0.CO%3B2-6
We report the isolation of molluscan (Lymnaea stagnalis) partial complementary DNAs (cDNAs) that encode two new putative glutamate-gated cation-channel polypeptides. These pro...
GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness
Book Chapter
Darlison, M. G., & Harvey, R. J. (1996)
GABA-, Glycine-, and Glutamate-Gated Channels and Their Possible Involvement in Neurological and Psychiatric Illness. In Molecular Biology of Membrane Transport Disorders, 169-180. Springer Verlag. https://doi.org/10.1007/978-1-4613-1143-0_9
Rapid chemical communication between cells in the vertebrate central nervous system is mediated by ligand-gated ion-channel receptors (also called ionotropic receptors), which...
Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis
Journal Article
Hicks, A. A., Bailey, M. E., Riley, B. P., Kamphuis, W., Siciliano, M. J., Johnson, K. J., & Darlison, M. G. (1994)
Further Evidence for Clustering of Human GABAA Receptor Subunit Genes: Localization of the α6-Subunit Gene (GABRA6) to Distal Chromosome 5q by Linkage Analysis. Genomics, 20(2), 285-288. https://doi.org/10.1006/geno.1994.1167
GABAA receptors are hetero-oligomeric ion-channel complexes that are composed of combinations of α, β, γ, and δ subunits and play a major role in inhibitory neurotransmission ...
Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit
Journal Article
Glencorse, T. A., Darlison, M. G., Barnard, E. A., & Bateson, A. N. (1993)
Sequence and Novel Distribution of the Chicken Homologue of the Mammalianγ-Aminobutyric AcidAReceptorγ1 Subunit. Journal of Neurochemistry, 61(6), 2294-2302. https://doi.org/10.1111/j.1471-4159.1993.tb07473.x
cDNAs have been cloned that encode the chicken (Gallus domesticus) γ-aminobutyric acidA receptor γ1 subunit, the mature sequence of which shares 90, 79, and 69% identity with ...
Rheological spectroscopy of concentrated colloidal dispersions
Journal Article
Williams, P., Williams, D., & Williams, R. (1993)
Rheological spectroscopy of concentrated colloidal dispersions. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 77(1), 75-79. https://doi.org/10.1016/0927-7757%2893%2980101-j
A new instrument, the virtual gap rheometer (VGR) for the determination of dynamic shear moduli (and mechanical relaxation spectra therefrom) at frequencies above 100 Hz is de...
Alternative splicing increases GABA A receptor heterogeneity
Journal Article
Lasham, A., Bateson, A. N., & Darlison, M. G. (1991)
Alternative splicing increases GABA A receptor heterogeneity. Transactions, 19(1), 9S-9S. https://doi.org/10.1042/bst019009s
No abstract available.
The chicken GABA A receptor: heterogeneity of gamma subunits
Journal Article
Glencorse, T. A., Bateson, A. N., & Darlison, M. G. (1991)
The chicken GABA A receptor: heterogeneity of gamma subunits. Transactions, 19(1), 4S-4S. https://doi.org/10.1042/bst019004s
Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA
Journal Article
Fraser, S., Djamgoz, M., Usherwood, P., O'Brien, J., Darlison, M., & Barnard, E. (1990)
Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA. Molecular Brain Research, 8(4), 331-341. https://doi.org/10.1016/0169-328x%2890%2990047-h
Poly(A)+ messenger ribonucleic acid (mRNA) was extracted from leg muscles of the locust Schistocerca gregaria and injected into oocytes of Xenopus laevis. After 5–10 days incu...