Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity
Journal Article
Poulet, B., Liu, K., Plumb, D., Vo, P., Shah, M., Staines, K., …Bou-Gharios, G. (2016)
Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity. PLOS ONE, 11(12), https://doi.org/10.1371/journal.pone.0167971
Bone development and length relies on the growth plate formation, which is dependent on degradative enzymes such as MMPs. Indeed, deletion of specific members of this enzyme f...
Opportunistic screening to detect atrial fibrillation in Aboriginal adults in Australia
Journal Article
Gwynne, K., Flaskas, Y., O'Brien, C., Jeffries, T. L., McCowen, D., Finlayson, H., …Freedman, B. (2016)
Opportunistic screening to detect atrial fibrillation in Aboriginal adults in Australia. BMJ Open, 6(11), https://doi.org/10.1136/bmjopen-2016-013576
Introduction There is a 10-year gap in life expectancy between Aboriginal and non-Aboriginal Australians. The leading cause of death for Aboriginal Australians is cardiovascul...
A role for the metalloprotease invadolysin in insulin signaling and adipogenesis
Journal Article
Chang, C., Abhinav, K., Di Cara, F., Panagakou, I., Vass, S., & Heck, M. M. (2017)
A role for the metalloprotease invadolysin in insulin signaling and adipogenesis. Biological Chemistry, 398(3), 373-393. https://doi.org/10.1515/hsz-2016-0226
Invadolysin is a novel metalloprotease conserved amongst metazoans that is essential for life in Drosophila. We previously showed that invadolysin was essential for the cell c...
The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures
Journal Article
Houston, D. A., Myers, K., MacRae, V. E., Staines, K. A., & Farquharson, C. (2016)
The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures. Calcified Tissue International, 99(5), 510-524. https://doi.org/10.1007/s00223-016-0176-9
Sustained exposure to high levels of parathyroid hormone (PTH), as observed in hyperparathyroidism, are catabolic to bone. The increase in the RANKL/OPG ratio in response to c...
Models of ex vivo explant cultures: applications in bone research
Journal Article
Marino, S., Staines, K. A., Brown, G., Howard-Jones, R. A., & Adamczyk, M. (2016)
Models of ex vivo explant cultures: applications in bone research. BoneKEy Reports, 5, 818. https://doi.org/10.1038/bonekey.2016.49
Ex vivo explant culture models are powerful tools in bone research. They allow investigation of bone and cartilage responses to specific stimuli in a controlled manner that cl...
Antiviral host defence peptides.
Book Chapter
Sousa, F. H., Casanova, V., Stevens, C., & Barlow, P. G. (2016)
Antiviral host defence peptides. In R. M. Epand (Ed.), Host Defense Peptides and Their Potential as Therapeutic Agents, 57-94. Springer Verlag. https://doi.org/10.1007/978-3-319-32949-9_3
The on going global mortality and morbidity associated with viral pathogens highlights the need for the continued development of effective, novel antiviral molecules. The anti...
Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model
Journal Article
Staines, K. A., Madi, K., Mirczuk, S. M., Parker, S., Burleigh, A., Poulet, B., …Pitsillides, A. A. (2016)
Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model. Arthritis and Rheumatology, 68(4), 880-891. https://doi.org/10.1002/art.39508
OBJECTIVE:
To explore whether aberrant transient chondrocyte behaviors occur in the joints of STR/Ort mice (which spontaneously develop osteoarthritis [OA]) and whether they a...
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
Journal Article
Houston, D. A., Staines, K. A., MacRae, V. E., & Farquharson, C. (2016)
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification. Journal of Visualized Experiments, 118(118), https://doi.org/10.3791/54978
The fundamental process of endochondral ossification is under tight regulation in the healthy individual so as to prevent disturbed development and/or longitudinal bone growth...
E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models
Journal Article
Staines, K. A., Prideaux, M., Allen, S., Buttle, D. J., Pitsillides, A. A., & Farquharson, C. (2016)
E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models. Journal of Cellular Physiology, 231(6), 1392-1404. https://doi.org/10.1002/jcp.25282
The transmembrane glycoprotein E11 is considered critical in early osteoblast–osteocyte transitions (osteocytogenesis), however its function and regulatory mechanisms are stil...
Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing
Journal Article
Zaman, G., Farquharson, C., Staines, K. A., Newton, P. T., Dudhia, J., Chenu, C., …Dhoot, G. K. (2016)
Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing. Histochemistry and Cell Biology, 145(1), 67-79. https://doi.org/10.1007/s00418-015-1365-8
SULF1/SULF2 enzymes regulate cell signalling that impacts the growth and differentiation of many tissues. To determine their possible role in cartilage and bone growth or repa...